mEGFMurine Epidermal Growth Factor (also seen as EGF)
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[39] and cultured in a 1: 1 mixture of DMEM: Ham's F12 medium containing 10 nM sodium selenite, 1.2 g/L sodium bicarbonate, 10 [micro]g/mL transferrin, 1% of CDL, and 50 ng/mL mEGF in fibronectin-coated 25 [cm.sup.2] T-flasks.
All cell lines were grown on fibronectin coated dishes, in an expansion medium supplemented with mPDGF-BB, mEGF, and containing 2% FBS.
MEGF), and University of Tennessee, Memphis, Memphis, Tennessee (DAJ).