Therefore, comparing our findings to those described by Simons and colleagues , we found that MTP1
is located in quadrant A and MTP 2 and MTP 3 are located in quadrants C and D (Figure 2), respectively.
Metal Tolerance Protein 1 (MTP1) involved in accumulation of Zn and not of cadmium is considered one of the potential candidates to be used for this purpose (Drager et al., 2004; Desbrosses-Fonrouge et al., 2005).
Previously, it was shown that MTP1 of poplar forms oligomers when expressed in yeast as well as in planta (Blaudez et al., 2003).
At the same time as a control, AhMTP1-A2 was also co-expressed with another gene involved in Zn tolerance but not showing sequence similarity to MTP1, AhPDF1.1 (Mirouze et al., 2006).
Thus, our work points to the requisite that when developing Zn biofortification approach for cereals using AhMTP1, compatibility between exogenous and endogenous MTP1 of cereals must be checked.
Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane, which mediates Zn detoxification and drives leaf Zn accumulation.
The SWM was pressed to the skin three times at each location (first toe, MTP1, and MTP5).
The vibrating insoles consisted of a cork sole with three built-in piezoelectric elements (piezo element EPZ35MS29, 35 mm diameter, Karl/Heinz Mauz GMBH; Ostfildern, Germany) at MTP1, MTP5, and the heel; the sole was covered with a thin leather layer.