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Assessment of the efficacy of an IgM- ELISA and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis.
Assessment of the efficacy of IgM ELISA and Microscopic agglutination test (MAT) in the diagnosis of leptospirosis.
Microscopic Agglutination Test (MAT): was performed by standard procedure (13) using the following twelve serovars.
Laboratory-confirmed diagnosis of leptospirosis was defined as a 4-fold rise in titer between paired samples or a single titer of [greater than or equal to] 800 in the microscopic agglutination test (MAT) (11) or a positive result on the immunoglobulin M (IgM) ELISA (Bio-Manguinhos, Rio de Janeiro, Brazil) (13).
For antibody detection, microscopic agglutination test was conducted with a panel of 12 leptospiral serovars; a titer [greater than or equal to] 100 was considered positive (Table 1).
The microscopic agglutination test was performed with a panel of 24 antigens belonging to 17 serogroups of Leptospira spp.
Leptospirosis was diagnosed by polymerase chain reaction (PCR) targeted to the flagellin gene (flaB) and confirmed serologically with convalescent-phase serum by microscopic agglutination test.
The referent method for serologic diagnosis of leptospirosis is the microscopic agglutination test (MAT).