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References in periodicals archive ?
Assessment of the efficacy of an IgM- ELISA and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis.
Microscopic agglutination test (MAT) is the gold standard for detection of antibodies of leptospira organism in dogs (OIE, 2008).
Serogroup/serovar specific tests: Microscopic agglutination test. Serovar specific ELISA.
Leptospirosis case are diagnosed by serology, method is the microscopic agglutination test (MAT), in which live antigens representing different serogroups of leptospires are reacted with serum samples and then examined by dark field microscopy for agglutination[7].Diagnostic application of the MAT is limited by the relatively low sensitivity when acute serum samples are tested [38].IgM antibodies are detectable after about the fifth day of illness.
Secondly, pertaining to the serological test that has been used, although the microscopic agglutination test (MAT) is the reference method for epidemiological serosurveys of leptospirosis, it has several drawbacks.
Accurate diagnosis of leptospirosis has been hampered by the difficulty of the serologic testing with the gold standard microscopic agglutination test. The high sensitivity and specificity of the newer rapid assays in early infection, their ease of use, and stability in field settings probably will result in earlier and more accurate diagnosis of leptospirosis.
An increase in antibody titers in paired serum samples was observed against the isolate (reciprocal titers 50 and 200 in acute- and convalescentphase samples, respectively), according to microscopic agglutination test (3).