Changes of the expression levels of this selection of genes are very small when induced by n-VLDL, varying by a larger extent if VLDL were preliminarily oxidised (see Table 1).
In this frame, the 24 h cell incubation with n-VLDL is clearly less effective.
The protein expression of eNOS was determined by Western blot analysis in HUVEC incubated with n-VLDL or ox-VLDL.
Control cells exhibited a ~60% phosphorylation at Ser1177 (P-Ser1177), and incubation with n-VLDL (both 75 and 140 [micro]g m[L.sup.-1]) did not induce significant changes.
The [O.sub.2] consumption of HUVEC following treatment with n-VLDL or ox-VLDL was measured oxigraphically.
Typical traces are reported in Figure 4(a), where HUVEC untreated or incubated with n-VLDL or ox-VLDL, both 140 [micro]g m[L.sup.-1], were allowed to consume [O.sub.2] in the presence and absence of selective activators or inhibitors of specific respiratory chain complexes [49, 50].
The basal respiratory control ratio, RCR, was also affected by the VLDL incubation; it decreased from 1.74 to 1.50 and 1.40, upon treatment with n-VLDL and ox-VLDL, respectively (see Figure 4(c)).
HUVEC were incubated 24 h with n-VLDL (grey) orox-VLDL (black), both 140 [micro]g/mL.
HUVEC were incubated 24 h with (75 and 140 [micro]g/mL) n-VLDL or ox-VLDL and then assayed by Western blot with anti-eNOS antibodies (see Materials and Methods).
Assays were carried out following 24 h incubation of HUVEC with n-VLDL or ox-VLDL (75 and 140 [micro]g/mL).