Plasmid pVEN-3 encoding major outer membrane polymorphic proteins (OMPs) and FlaB from Vibrio vulnificus was constructed by sequentially inserting chlamydial PmpD and omp1
sequences as well as the FlaB sequences into plasmid pVAX1.
Validation of Roche COBAS Amplicor assay for detection of Chlamydia trachomatis in rectal and pharyngeal specimens by an omp1
Thus, chlamydial outer membrane proteins (OMP1
, OMP2 and PorB) were expressed in the periplasmic space using the periplasmic targeting vector pMALp2x.
Serotyping and genotyping of genital Chlamydia trachomalis isolates reveal variants of serovars Ba, G, and J as confirmed by omp1
nucleotide sequence analysis.
trachomatis spp., all stored rectal specimens were analyzed by using a nested omp1
PCR-restriction fragment length polymorphism assay.
Evidence for numerous omp1
alleles of porcine Chlamydia trachomatis and novel chlamydial species obtained by PCR.
Serosubtyping of serogroup C isolates in this outbreak showed variation in the class 1 outer membrane protein (OMP1
The sequence of AB induction is as follows: (1) an infected person is asymptomatic; (2) AB is resistant to antibiotics; (3) Ct DNA can be detectable but the pathogen cannot be isolated; (4) complications such as chronic pelvic inflammation, ectopic pregnancy, and tubal infertility may occur; (5) RB stops the fission and converts to an abnormal and large AB; (6) expression of OMP1
gene decreases and hsp60 gene increases; and (7) when these condition (s) such as antibiotic treatment, IFN, and malnutrition are removed, AB converts to EB.
All studies described in-house methods for LGV identification: 2 used a real-time PCR targeting the polymorphic membrane protein H gene (17,26): 1 used nested PCR and restriction fragment length polymorphism (RFLP) analysis targeting the major outer membrane protein gene (25): 1 used real-time multiplex PCR including LGV (31): 1 used PCR amplification of the outer membrane protein 1 (omp1
) gene followed by RFLP analysis (27); 1 used omp1
gene sequencing (29): 1 used real-time polymorphic membrane protein H gene PCR and omp1
gene sequencing (3); 1 used LGV-specific molecular serovar typing (28): and 1 used an in-house assay (30).
Usefulness of omp1
restriction mapping for avian Chlamydia psittaci isolate differentiation.