Similarly, administering an increased amount of ttIL12 pDNA
did not increase the efficacy of ttIL12.
These developments has spurred the production of high amount, high quality pharmaceutical grade pDNA
. Large-scale production of plasmid DNA (pDNA
) for medical use is however a new field in biopharmacy.
prime/poxvirus boost vaccine approach has been developed since 1998 by the EuroVacc programme and is currently the focus of the CAVD's Poxvirus T-cell Vaccine Discovery Consortium.
The first contract, awarded in April 2000, focused on adjuvant-enhanced plasmid DNA (pDNA
) and peptide-based vaccines.
Aldehyde sodium alginate modified [Fe.sub.3][O.sub.4] magnetic particles coated cisplatin (SPION-CDDP) was constructed with the above cationic polymer to obtain composite carriers (FA-PEGPEI@SPION-CDDP), which then adsorbed TFPI-2 pDNA
via electrostatic interaction to obtain the FA-targeted, CDDP, and TFPI-2-coated nanocomposites (FA-PEG-PEI@SPIONCDDP-TFPI-2).
"This is the biggest PDNA
that the government will conduct," said Ana C.
The authors used a ternary formulation of mRNA or pDNA
coding for the reporter gene EGFP (Enhanced Green Fluorescent Protein) complexed with PEGylated histidylated poly-L-Lysine and imidazole-rich liposomes, both of which promote endosomal escape [224, 225].
Plasmid DNA (pDNA
) was dissolved in TE buffer, and the concentration was determined at 260 nm using a NanoDrop 2000c spectrophotometer (Thermo Scientific, USA).
In our previous study, we constructed glycyrrhetinic acid-modified stealth cationic liposomes loaded with pDNA
and they were found to transfect human hepatocellular carcinoma cell line HepG2 with high efficiency .
"I don't think it's protocol, I don't think it's SOP (standard operating procedure); I don't think that it's regular that it will take six months to come up with a post disaster needs assessment (PDNA
) report," he said.