pGHRPorcine Growth Hormone Receptor
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As illustrated in Figure 2B and 2C, after pGH treatment, pGHR was primarily localized in the cytoplasm and nucleus.
In addition, we also performed EM experiments to observe pGHR nuclear localization, and as shown in Figure 2E (see indicated arrows), pGHR was detected in cell nuclei and the nuclear membrane.
To determine whether pGHR nuclear localization is specifically induced by pGH, a pGHR-specific antibody and control antibody (B32 developed in a previous study and demonstrated to compete with pGH for GHR binding [11]) were pre-injected in the inferior vena cava for 30 min prior to pGH injection.
In the present study, to our knowledge, we demonstrated the first exploration of pGHR nuclear translocation induced by pGH in vivo in domestic animals.
Almost all tissues express pGHR, suggesting that pGH has potential effects on all tissues.
Indeed, the mechanism(s) of pGHR nuclear translocation remains to be fully understood.
Taken together, the results of the present study provide the first evidence that pGH can induce pGHR nuclear translocation in vivo, indicating that pGHR not only functions in the cell membrane but also in cell nuclei.
Growth hormone (GH)-specific induction of the nuclear localization of porcine growth hormone receptor (pGHR) in the porcine hepatocytes.
Next, we further identified the reaction of A98 with pGHR (full-length pGHR) expressed on target cells.
In this study, we prepared and characterized a monoclonal antibody against pGHR, denoted by AN98, which not only specifically binds to pGHR but also serves as a potential pGHR antagonist.
Currently, there are no reports about pGHR-specific antagonists or whether the above-mentioned GHR antagonists targeting different species can recognize pGHR. Therefore, in this study, we developed a pGHR-specific antibody antagonist, AN98, which effectively inhibits GH-induced signaling (Figures 4 and 5), and inhibits IGF-1 secretion in primary hepatocytes.
In addition, AN98 shares common GHR-binding epitopes with pGH; therefore, AN98 could inhibit pGH binding to pGHR. The current findings suggest that AN98 is as a pGHR-specific antagonist, which is a useful tool for pGHR-related research in domestic pig.