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This was cloned into pJET cloning vector and transformed in TOP10 competent cells, and the plasmids from the positive colonies were double digested with EcoRI HF and SpeI-HF.
We extracted PCR products after electrophoresis from a 0.7% agarose gel, purified them by using a MEGAquickspin Total Fragment DNA Purification Kit (iNtRON Biotechnology, http://jhscience.com/index.php?manufacturers_ id=1), and cloned them into a pJET 1.2 blunt vector by using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, https://www.thermofisher.com/us/en/home.html).
(OTC: PJET) said that for the three months ending March 31, 2015, the company reported total gross aviation related revenues of USD1.75 million, as compared to USD1.71 million for the same period in 2014.
PCR products obtained from serum and fecal samples were cloned into pJET Vector (Thermo Scientific); 3 positive clones for each sample were sequenced.
He referred to IoBM research journals, HEC approved, top-rated, X-category standing for Pakistan Business Review (PBR) and Pakistan Journal of Engineering, Technology and Science (PJETS) elevation to Y-category in 2018.
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