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Related to polyacrylamide: polyacrylamide gel electrophoresis
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References in periodicals archive ?
The objective of this experiment was to assess the effect of different formulations of polyacrylamide with varying molecular weight and charge density on the turbidity of water.
2015) Highly sensitive fluorescent stain for detecting lipopolysaccharides in sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Two-year follow-up of an open-label multicenter study of polyacrylamide hydrogel (Bulkamid) for female stress and stress-predominant mixed incontinence.
Green SV, Stott DE, Norton LD, Graved JG (2000) Polyacrylamide molecular weight and charge effects on infiltration under simulated rainfall.
Presently two-dimensional gel electrophoresis (2-DE), with one dimension on isoelectric focusing (IEF) and other on sodium dodecyle sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and vice versa, is mostly employed in such analysis.
Meanwhile, the polyacrylamide chains form a grid-like structure that bonds covalently (very tightly) with the alginate chains.
Polyacrylamide was used as coagulant aid which aid in settling the flocs readily.
By contrast, flocculation employs a high molecular weight polymer, usually polyacrylamide (PAM), to act as a microscopic spider web, entangling the fine particles and forcing them to sink.
1990) and the protein in the supernatant was collected and separated on a sodium dodecyl sulphate polyacrylamide gel electro-pheresis (SDS-PAGE).
The apparent molecular weight of the peptide was approximated by tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis.
The purpose of this study was to optimize fermentation conditions (pH, incubation periods and glucose concentrations) for maximum production of peptide antibiotics from isolated Bacillus subtilis immobilized in polyacrylamide gel and screened for the production of antibiotics by shake flask fermentation at 30oC by checking activity against Micrococcus luteus (ATCC#10240) through antibiotics diffusion assay.
The authors compare the different protein purification procedures, walk through the steps in planning a lipase purification strategy by affinity chromatography, and highlight the importance of assessing purified proteins by polyacrylamide gel electrophoresis.