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References in periodicals archive ?
Xiao, "Quantitative haplotyping of PCR products by nonsynchronous pyrosequencing with di-base addition," Analytical and Bioanalytical Chemistry, vol.
With this study, microbial diversity has been determined with culture-dependent and culture-independent (454 pyrosequencing) for the first time in some of the Bursa's hot springs using a holistic approach.
Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients.
Pyrosequencing reactions were performed using Pyromark Gold Q96 reagents (Qiagen) according to the manufacturer's recommendations.
The amplicons from the validation PCR and the initial pyrosequencing PCR reactions were purified using the Wizard DNA Purification Kit (Promega, Madison, WI, USA).
This study was carried out to investigate the effect of different DNA extraction procedures and primer sets on pyrosequencing results regarding the composition of bacterial communities in the ileum of piglets.
Researchers analyzed three new tests: pyrosequencing, a DNA sequencing technique; the HAIN line probe test, a commercial test that detects genetic mutations in the bacteria; and the microscopic observation drug susceptibility (MODS) test, which screens samples under the microscope.
Nine tenders for the supply of different scientific instruments including (a) highly flexible, comprehensive, real-time PCR platform with digital PCR (open array block) including digital plate loading system (an AccuFill system), (b) automated nucleic acid extractor, (c) automated PCR setting equipment, (d) pyrosequencing technology for genetic analysis by real-time sequencing of PCR templates, (e) gel documentation system with built in camera, (f) gradient thermo cycler + tools for the same, (g) a vacuum concentrator, (h) a refrigerated (keypad) centrifuge including rotor for spin columns and two additional rotors, also (i) an upright ultra low temperature deep freezer (up to minus 86 grade C).
The researcher used pyrosequencing, a relatively new method of rapidly analyzing bacterial DNA markers, to classify the bacteria into different taxonomic groups.
"This is one of the first studies to document such diversity using the pyrosequencing technique (a large scale DNA sequencing determination technique) on colostrum samples on the one hand, and breast milk on the other, the latter being collected after one and six months of breastfeeding," co-authors, Maria Carmen Collado from the Institute of Agrochemistry and Food Technology (IATA-CSIC) and Alex Mira, researcher at the Higher Public Health Research Centre (CSISP-GVA), said.
Here, we analyzed bacterial communities across the ants (Hymenoptera: Formicidae) using 16S rRNA tag encoded amplicon pyrosequencing (454 pyrosequencing) to survey for infection patterns with potential parasitic microbes.