rhSCFRecombinant human Stem Cell Factor
References in periodicals archive ?
Botana et al., "The human recombinant c-kit receptor ligand, rhSCF, induces mediator release from human cutaneous mast cells and enhances IgE-dependent mediator release from both skin mast cells and peripheral blood basophils," The Journal of Immunology, vol.
The purified [CD133.sup.+] cells were suspended at 5 x [10.sup.5] cells/mL in StemSpan medium (Stem Cell Technologies, Vancouver, British Columbia, Canada) supplemented with 100 ng/mL human recombinant stem cell factor (rhSCF; R&D Systems, Abingdon, UK), 50 ng/mL human recombinant interleukin-6 (rhIL-6; R&D Systems), 1 ng/mL human recombinant interleukin-3 (rhIL-3; PeproTech, London, UK), and 100 [micro]g/mL penicillin/streptomycin (GIBCO BRL, Grand Island, NY, USA) and grown for 3 weeks, after which rhIL-3 was omitted from the culture media.
Poulsen (National University Hospital, Copenhagen, Denmark) for 24 hr at 37[degrees]C in StemSpan medium (Stem Cell Technologies) supplied with 100 ng/mL rhSCF and 50 ng/mL rhIL-6.