Thus, given a choice between conjugating an antibody or scFv
antibody or aptamer to a liposome, it would seem that lower molecular weight ligands would enable greater penetration of the NP within the tumor.
Phyre2 was applied to build the 3D structure of the anti-LOX-1 scFv
(http://www.sbg.bio .ic.ac.uk/phyre2/html/page.cgi?id=index) .
In this study, we expressed anti-ICAM-1 scFv
at a high level in the form of inclusion bodies in Escherichia coli.
The package heads straight for the cell nucleus where a different chemical environment breaks the bond, freeing ScFv
18-2 to attack the regulatory region of DNA-dependent protein kinase, an enzyme essential to DNA repair.
 Nonstandard abbreviations: GIP, glucose-dependent insulinotropic peptide; GLP-1, glucagon-like peptide-1; DPP4, dipeptidyl peptidase 4; VH, Ig heavy chain variable region; VL, Ig light chain variable region; scFv
: single chain fragment variable; CDR, complementarity-determining region; MSD, MesoScale Discovery; TBST, Tris buffered saline + tween; GIPR: GIP receptor; OGTT, oral glucose tolerance test.
A biblioteca de fragmentos de anticorpos scFv
previamente preparada com a estirpe H120 de referencia vacinal do VBI (CAETANO, et al., 2009) foi recuperada por meio do crescimento das celulas transformantes ate a fase exponencial, em que foram infectadas com o fago auxiliar M13K07 (GE Healthcare) numa multiplicidade de infeccao (moi) de 5:1, sendo seguidas as recomendacoes de SAPATS et al.
These are fusion proteins composed of an antigen recognition domain--typically an antibody single-chain variable fragment (scFv
)--fused to a variety of intracellular signaling domains.
(2006) Quality enhancement of the non-immune phage scFv
library to isolate effective antibodies.
High-level expression and characterization of an anti-[VEGF.sub.165] single-chain variable fragment (scFv
) by small ubiquitin-related modifier fusion in Escherichia coli.
CAR construct typically uses a single chain variable fragment, or scFv
, to recognize a protein of interest.
Two types of antibodies against 5,-nucleotidase were used in order to compare antigen affinity: a commercially purified anti-CD73 monoclonal antibody (Ecto-5,-nucleotidase, 5F/B9, BD Pharmingen) (namely, CD73) and single chain fragment Fv monoclonal antibody obtained by phage display method (namely, ScFv
Using LPH-NP (liposome-polycation-hyaluronic acid) nanoparticles targeted by postinsertion of DSPE-PEG-GC4 (scFv
selected by phage display against ovarian tumors ), they codelivered 3 different siRNA and one miRNA and obtained a 80% decrease in tumor load after treatment.