scFvSingle-Chain Variable Fragment (antibody)
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Thus, given a choice between conjugating an antibody or scFv antibody or aptamer to a liposome, it would seem that lower molecular weight ligands would enable greater penetration of the NP within the tumor.
Phyre2 was applied to build the 3D structure of the anti-LOX-1 scFv ( [15].
In this study, we expressed anti-ICAM-1 scFv at a high level in the form of inclusion bodies in Escherichia coli.
The package heads straight for the cell nucleus where a different chemical environment breaks the bond, freeing ScFv 18-2 to attack the regulatory region of DNA-dependent protein kinase, an enzyme essential to DNA repair.
[2] Nonstandard abbreviations: GIP, glucose-dependent insulinotropic peptide; GLP-1, glucagon-like peptide-1; DPP4, dipeptidyl peptidase 4; VH, Ig heavy chain variable region; VL, Ig light chain variable region; scFv: single chain fragment variable; CDR, complementarity-determining region; MSD, MesoScale Discovery; TBST, Tris buffered saline + tween; GIPR: GIP receptor; OGTT, oral glucose tolerance test.
A biblioteca de fragmentos de anticorpos scFv previamente preparada com a estirpe H120 de referencia vacinal do VBI (CAETANO, et al., 2009) foi recuperada por meio do crescimento das celulas transformantes ate a fase exponencial, em que foram infectadas com o fago auxiliar M13K07 (GE Healthcare) numa multiplicidade de infeccao (moi) de 5:1, sendo seguidas as recomendacoes de SAPATS et al.
These are fusion proteins composed of an antigen recognition domain--typically an antibody single-chain variable fragment (scFv)--fused to a variety of intracellular signaling domains.
(2006) Quality enhancement of the non-immune phage scFv library to isolate effective antibodies.
High-level expression and characterization of an anti-[VEGF.sub.165] single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli.
CAR construct typically uses a single chain variable fragment, or scFv, to recognize a protein of interest.
Two types of antibodies against 5,-nucleotidase were used in order to compare antigen affinity: a commercially purified anti-CD73 monoclonal antibody (Ecto-5,-nucleotidase, 5F/B9, BD Pharmingen) (namely, CD73) and single chain fragment Fv monoclonal antibody obtained by phage display method (namely, ScFv) [16].
Using LPH-NP (liposome-polycation-hyaluronic acid) nanoparticles targeted by postinsertion of DSPE-PEG-GC4 (scFv selected by phage display against ovarian tumors [241]), they codelivered 3 different siRNA and one miRNA and obtained a 80% decrease in tumor load after treatment.