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Related to single-stranded DNA: B DNA
ssDNASingle-Stranded DNA (Deoxyribonucleic Acid)
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Several investigators have used exonuclease digestion of the complementary strand to produce single-stranded DNA targets for hybridizations (8-16).
The RNase H cleaves the RNA portion of the heteroduplex at one end of the double-stranded cDNA, thus generating a unique partial duplex cDNA with a single-stranded DNA tail at the 3' end of the second-strand cDNA.
Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules.
Evidence that these two bands were single-stranded DNA was that they were sensitive to mung-bean nuclease, which specifically digests single-stranded DNA.
For example, a single-stranded DNA segment consisting of the base sequence TAGCC will stick to a section of another strand made up of the complementary sequence ATCGG.
Bound biotinylated single-stranded DNA was generated using protocols recommended by the manufacturer (PSQTM HS 96A System Sample Preparation Kit; Pyrosequencing [micrAB).
As the basis of their proposed molecular computer, Roweis and his coworkers use two different types of single-stranded DNA. Memory strands consist of long sequences of bases subdivided into segments of a standard length that represent individual memory units.
Competition studies demonstrated inhibition of binding by nucleosomes, 200/400-bp DNA, salmon sperm DNA, and, to a lesser extent, single-stranded DNA. DNase treatment removed 95.3% [+ or -] 2.3% of the cell-associated radioactivity at 37[degrees]C.
Chen and Seeman started with two long and eight short pieces of single-stranded DNA, which they had designed on a computer and then synthesized.
Accordingly, we have constructed a LOCI microscope and used it to demonstrate sensitive detection of analytes in small volumes for three types of assays of potential interest in arrays: detection of a single-stranded DNA fragment, detection of a double-stranded DNA amplicon, and an immunoassay for a protein.
Scientists in this area are experimenting with small pieces of single-stranded DNA called oligonucleotides, or "oligos." They can synthesize these in the laboratory, assembling sequences of single-stranded DNA complementary to a gene sequence they want to suppress.
The presence of multiple bands for both alleles is likely caused by alternative conformations of single-stranded DNA. The non-denatured, double-stranded PCR product migrated much faster than the single-stranded forms and therefore is not seen in Fig.
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