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snoRNASmall Nucleolar RNA
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The PWS/AS imprinted domain is highly complex, containing a large number of C/D box small nucleolar RNAs (snoRNAs) and two ICRs: Prader-Willi Syndrome Imprinting Control (PWS-IC) and Angelman Syndrome Imprinting Control (AS-IC) (Wu et al.
Small non-coding RNAs (sncRNAs) mainly consist of microRNAs (miRNAs) and small nucleolar RNAs (snoRNAs) (8).
For loading control, the nylon membrane was reprobed with the 5'-[[[gamma]-.sup.32]P]-U6 snoRNA probe (lower panel).
Facilitated by next generation sequencing technologies, a complex compositional profile of salivary RNA molecules has emerged, encompassing mRNAs, microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), etc.
Recently, mutations in RNAse mitochondrial RNA processing RNA (RMRP), a noncoding SnoRNA that functions itself without being translated into a protein, have been connected to disorders mostly exhibiting skeletal dysplasia.
And the third group was composed of: sequences of snoRNA, collected from snoRNA-LBME-db database (; precursor miRNA sequences, extracted from miRBase (, version 21); and sequences of tRNA, extracted from genomic tRNA database (
In this regard, noncoding RNAs (ncRNAs) [5] in the blood of cancer patients, comprising small nucleolar RNA (snoRNA), microRNA (miRNA), piwi-associated RNA, small Cajal body--specific RNA (scaRNA), and small nuclear RNA (snRNA), have been suggested as novel biomarkers for noninvasive cancer diagnosis.
Then, the clean reads were compared against the non-coding (ncRNAs) (rRNAs, tRNAs, snRNAs, and snoRNA) deposited in the NCBI GenBank and the Rfam databases using BLAST to annotate the small RNA sequences.
It's been difficult to pick up the scent of snoRNA genes, Eddy says.
Additionally, up-to-date human snoRNA annotations were taken from literature [23].
Using "blastn" program with command "blastnshort task," we separated the small RNAs that matched other noncoding sequences, such as ribosomal RNA (rRNA), small nuclear RNA (snRNA), small nucleolar RNA (snoRNA), and transfer RNA (tRNA).