When they treated the bacteria with enzymes to free up the ssDNA
, they could isolate it, and then fold it into its target structure.
Absorption spectra of complex in the presence of SSDNA
are shown in Fig.
Lazarowitz, 1992) need for accumulation of ssDNA
Phylogenetic analysis of REP sequences showed that HV-CV1 and its closest homologue, an ssDNA
circular virus of unknown taxon discovered in an Antarctic shelf pond, are distantly related to other CRESS-DNA viruses (online Technical Appendix Figure 2).
To initiate in vitro selection, 5 nmol of the ssDNA
pool was diluted in 1000 [micro]l of binding buffer, denatured by heating at 95[degrees]C for 5 min and snap cooled on ice for 15 min.
The DNA SMART ChIP-Seq Kit makes it possible to further expand the benefits of SMART technology into other NGS applications, allowing the company to address the needs of those researchers working with low inputs of either dsDNA or ssDNA
for NGS applications, Lou added.
Detection of antibodies (Stern et al, 2007), ssDNA
(Kim et al, 2007), virus (Patolsky et al.
For instance, curcumin might act as inhibitor of the binding of RecA protein to the ssDNA
induced by levofloxacin.
Instead, in vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases, which act to locally unwind dsDNA to create small ssDNA
regions suitable for polymerase activity.
The major biochemical function of RPA is to bind to ssDNA
Maxwell (1994) Whitefly transmission and efficient ssDNA
accumulation of bean golden mosaic geminivirus requires functional coat protein.
Pre-programmed Applications : dsDNA, ssDNA
, RNA, and Oligonucleotides, Protein UV (A280), Protein Dye, Bradford, Lowry, BCA, Biuret, Cell density